Alexa Fluor® 647 Anti-ATPB antibody [3D5] (ab197649) (2024)

Mouse Monoclonal ATPB antibody - conjugated to Alexa Fluor® 647. Mitochondrion marker. Suitable for ICC/IF and reacts with Human samples.

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Alternative names=ATP synthase F1 subunit beta, ATPSB, ATP5F1B, ATP5B, ATPMB

Related conjugates and formulations

  • ab14730Unconjugated Anti-ATPB antibody [3D5] - Mitochondrial Marker
  • ab197904ConjugatedAlexa Fluor® 488 Anti-ATPB antibody [3D5] - Mitochondrial Marker
  • ab197905Conjugated HRP Anti-ATPB antibody [3D5] - Mitochondrial Marker
Isotype

IgG1

Host species

Mouse

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Consider this alternative

Reactivity data

Select an application

Product promise

Tested

Tested

Expected

Expected

Predicted

Predicted

Not recommended

Not recommended

ICC/IF
Human

Tested

Mouse

Predicted

Rat

Predicted

Caenorhabditis elegans

Predicted

Cow

Predicted

Monkey

Predicted

Images

ICC/IF

Tested

Tested
SpeciesDilution infoNotes

Species

Human

Dilution info

1/50

Notes

This product gave a positive signal in HepG2 cells fixed with 100% methanol (5 min).

Predicted

Predicted
SpeciesDilution infoNotes

Species

Mouse, Rat, Cow, Caenorhabditis elegans, Monkey

Dilution info

-

Notes

-

Target data

ATP5F1B

Recommended products

Mouse Monoclonal ATPB antibody - conjugated to Alexa Fluor® 647. Mitochondrion marker. Suitable for ICC/IF and reacts with Human samples.

Alternative names=ATP synthase F1 subunit beta, ATPSB, ATP5F1B, ATP5B, ATPMB

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Clone number

3D5

Purity

IgG fraction

Light chain type

kappa

Concentration

Purification notes

Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

Tested

We have tested this species and application combination and it works. It is covered by our product promise.

Expected

We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.

Predicted

This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.

Not recommended

We do not recommend this combination. It is not covered by our product promise.

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

  • Visit the general protocols
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Alexa Fluor® 647 Anti-ATPB antibody [3D5] (ab197649) (2024)
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