Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449) (2024)

Mouse Monoclonal CPT1A antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 7 publications.

Alternative names=CPT1-L, Carnitine palmitoyltransferase 1A, CPT I, CPTI-L, CPT1, CPT1A

Related conjugates and formulations

  • ab128568Unconjugated Anti-CPT1A antibody [8F6AE9]
  • ab198494Conjugated HRP Anti-CPT1A antibody [8F6AE9]
Isotype

IgG2b

Host species

Mouse

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Flow Cyt (Intra)

Tested

Tested
SpeciesDilution infoNotes

Species

Human

Dilution info

5 µg/mL

Notes

ab171465 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Predicted

Predicted
SpeciesDilution infoNotes

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended

Not recommended

SpeciesDilution infoNotes

Species

Cow

Dilution info

-

Notes

ab171465 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF

Tested

Tested
SpeciesDilution infoNotes

Species

Human

Dilution info

5 µg/mL

Notes

-

Predicted

Predicted
SpeciesDilution infoNotes

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended

Not recommended

SpeciesDilution infoNotes

Species

Cow

Dilution info

-

Notes

-

Target data

CPT1A

Recommended products

Mouse Monoclonal CPT1A antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 7 publications.

Alternative names=CPT1-L, Carnitine palmitoyltransferase 1A, CPT I, CPTI-L, CPT1, CPT1A

Key facts

Isotype

IgG2b

Host species

Mouse

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

8F6AE9

Purification technique

Precipitation Ammonium Sulphate

Concentration

Purification notes

Purity is near hom*ogeneity as judged by SDS-PAGE. ab171449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product was previously marketed under the MitoSciences sub-brand.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

Tested

We have tested this species and application combination and it works. It is covered by our product promise.

Expected

We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.

Predicted

This species and application combination has not been tested, but we predict it will work based on strong hom*ology. However, this combination is not covered by our product promise.

Not recommended

We do not recommend this combination. It is not covered by our product promise.

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

  • Visit the general protocols
  • Visit troubleshooting
Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449) (2024)

FAQs

What does Alexa Fluor 488 stain? ›

Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching.

Is Alexa Fluor 488 the same as Fitc? ›

Alexa Fluor 488 has a spectrum almost identical to that of fluorescein isothiocyanate (FITC), but with extraordinary photostability. Because of this photostability, it has become a choice for fluorescent microscopy applications and has become popular in cytometry applications.

How long do you incubate Alexa Fluor 488? ›

Dilute Alexa Fluor® FluoroNanogoldTM to a concentration of 0.2 10 µg/mL (1 : 8 to 1 : 400 dilution) in PBS-Milk buffer and add 30 µL to cells; incubate for 30 minutes with occasional shaking.

What is Alexa Fluor used for? ›

g/mL Alexa Fluor IgG conjugate can be used for fluorophore- and biotin-labeled antibodies and tandem-labeled antibodies in immunohistochemical applications. Alexa dyes are also compatible with common fixatives such as paraformaldehyde and susceptible to typical quenchers such as trypan blue.

What is Alexa Fluor 488 equivalent to? ›

Table 3. Alexa Fluor Dye equivalents of common dyes
If you are usingTry this Alexa Fluor dye
Cy2, FITC (fluorescein)Alexa Fluor 488
Cy3, TRITC (rhodamine)Alexa Fluor 555
Rhodamine redAlexa Fluor 568
Texas RedAlexa Fluor 594
4 more rows

Is Alexa Fluor 488 an antibody? ›

Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488.

Is Alexa Fluor 488 fixable? ›

Dextran, Alexa Fluor™ 488; 10,000 MW, Anionic, Fixable.

Are Alexa Fluor dyes fixable? ›

Are Alexa Fluor® dyes compatible with common fixatives, buffers, permeabilizing agents like formaldehyde, formalin, paraffin, xylene, Triton® X-100, etc.? Alexa Fluor® dyes are compatible with Triton®, formaldehyde, and formalin.

How long do Alexa Fluor antibodies last? ›

2.2 Storing conjugated antibodies
Fluorescent labels, eg Alexa Fluor®, Dylight®, FITC, PEHRP
Short-term storageStore at +4°C short term (1-2 weeks)Store at +4°C short term (1-2 weeks)
3 more rows

How do you store Alexa Fluor 488? ›

Storage: SNAP-Surface AlexaFluor 488 should be stored at -20°C (long term) or at 4°C in the dark(short term, less than 4 weeks).

What is the correction factor for Alexa Fluor 488? ›

The correction factor (CF) for Alexa Fluor 488 is CF260 = 0.3, CF280 = 0.11. To request additional information and datasets for Alexa Fluor 488, please contactinfo@aatbio.com. Unless otherwise specified, correction factor (CF) values are typically measured in ddH2O or PBS.

What does Alexa Fluor 488 phalloidin stain? ›

Alexa Fluor® 488 Phalloidin allows researchers to fluorescently stain the cytoskeleton through the binding of phalloidin to F-actin. This product is intended for use on fixed and permeabilized samples due to the toxicity associated with phalloidin.

What does Alexa Fluor bind to? ›

Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

What is the fluorescent stain for E coli? ›

The fluorescent dye ethidium bromide stains Escherichia coli and Staphylococcus aureus in whole blood.

What fluorescent dye stains DNA? ›

SYBR Green I Nucleic Acid Gel Stain is an extremely sensitive fluorescent stain for detecting nucleic acids in both agarose and polyacrylamide gels, detecting as little as 60 pg of DNA.

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